![Fig. 6. Effect of Ro 31-8220 or dimethyl-BAPTA on collagen-induced fibrinogen receptor activation. / Collagen was added to aspirin-treated, washed human platelets that were pretreated with Ro 31-8220 (10 μM) or dimethyl-BAPTA (20 μM) or both in the presence of feedback inhibitors, and filtered through 10-μm filters. The filters were then probed with PAC-1 followed by HRP-conjugated secondary antibody, and the color was measured using OPD as the substrate. The adhesion was measured by counting of [3H]-oleic acid in platelets. Adhesion ranged from 23% to 32%. The ratio of PAC-1 binding (OPD reading) to fraction of adhesion was then expressed as relative PAC-1 binding. Each data point is the mean ± SE of 3 measurements using platelets from different donors.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3228/6/h80922502006.jpeg?Expires=1724147137&Signature=sYbxUgA-lQZwI3RvlM8--YQYBMNb0H5FQMdMadkQRkH2nmDcLbHGkxSleYOhCrvrzDZt0yKQxXiBrwhMIT5m5vOvSrZcINEf3ZWD~ceYRTIJ9thEs1SMstPgc10JgepqkJKZ0nVihSemA6RT~gsqSuud~BblSz-GKRBcMV14jOd1kYJNK~93hAqTpCM75SZ3NSHIPhW9eGDPeqYOBgj4VUAHgIaKBRkAGF9eNLZYn5kVjebWUtU48fzkjPjcXEu54wWS94KVK-ygNQ12BTKd4GvkwnXTClKTkVsjMqwt0XXW6gUxOotysOxRYtSGd92t~ul3UNmP354tN4sJlv9Egg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Ro 31-8220 or dimethyl-BAPTA on collagen-induced fibrinogen receptor activation.
Collagen was added to aspirin-treated, washed human platelets that were pretreated with Ro 31-8220 (10 μM) or dimethyl-BAPTA (20 μM) or both in the presence of feedback inhibitors, and filtered through 10-μm filters. The filters were then probed with PAC-1 followed by HRP-conjugated secondary antibody, and the color was measured using OPD as the substrate. The adhesion was measured by counting of [3H]-oleic acid in platelets. Adhesion ranged from 23% to 32%. The ratio of PAC-1 binding (OPD reading) to fraction of adhesion was then expressed as relative PAC-1 binding. Each data point is the mean ± SE of 3 measurements using platelets from different donors.
