Effect of C/EBP inhibition on induction of CD11b or PU.1 by G-CSF.

Inhibition of C/EBPs does not prevent induction of CD11b or PU.1 by G-CSF. (A) KαER/GR cells were cultured in G-CSF with and without 4HT, and the expression of CD11b was assessed by FACS analysis daily. Results from a representative experiment on days 0 and 5 are shown. (B) The 32D-KαER/GR2 cells were cultured in G-CSF without or with 4HT, and total cellular RNAs prepared on days 0, 2, 4, and 7 were subjected to Northern blotting for MPO, PU.1, and β-actin.