L3055 cells succumb to apoptosis at low plating densities.
(Upper panel) L3055 cells (wild-type; empty vector controls;bcl-2 transfected) were plated at numbers indicated in flat-bottom microwells containing 200 μL culture medium. DNA synthesis was assessed 5 days later by 3HTdr incorporation during a 4-hour pulse. Results represent the mean (± SE) of 3 separate experiments and are expressed as cpm per 1000 cells plated. (Lower panel) L3055 cells (wild-type; empty vector controls; bcl-2 transfected) were cultured at 105 or 104 per mL as indicated for 3 days and then stained with acridine orange to assess nuclear morphology and trypan blue to assess viability. Results are expressed as the mean percentage (± SE) apoptotic (filled bars) and viable cells (open bars) of 3 separate experiments.
