Fig. 3.
![Fig. 3. In the presence of TGF-β, HATGF-βRII-Δcyt–transduced CTLs continue to proliferate and grow normally in long-term cultures. / HATGF-βRII-Δcyt–transduced, nontransduced, and eGFP-transduced CTLs were harvested 2 weeks after transduction. From each group, 1 × 106 cells were stimulated weekly with LCLs and fed twice weekly with IL-2 ± 5 ng/mL TGF-β. The figures show results from one representative experiment. (A) This panel represents CTL cell numbers (× 106) recorded from weekly cell counts in the 3 CTL groups grown without TGF-β (■) and with TGF-β (▴). (B) This panel shows 3[H] thymidine uptake in nontransduced CTLs (black) and HATGF-βRII-Δcyt–transduced CTLs (gray) before addition of TGF-β then on days 4 and 12.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3179/6/h80922475003.jpeg?Expires=1724247447&Signature=SEgHUH8DsJzAQS4VnHroqIriY0ggZ2qVz2k8~7Lpz03C50reKQ1inUbQ-McKabWsiSos3pjXRyuv0efxQhGofG57SCTO~WdP6OpeEmPBydedr33ja4tHs-EwD3zr-ULIO3R~1bfYm9jmLN6akNgAslRaH8DcR~If9wF5QNzNmaV5maef2es1g1LXRp2s3qZi3DHqb0aGmXOKDQgF0HC7xANvA5bHSfTSys7adTaL3uu8OYSInI4~0VBjaEkuSEZALFHWDGFsOgv0oT1KNucyv6Rx~8mO3HTnO9LyR4NZ-YY1R-akqSab~bh-iB7bylrkz36U~IHfn81Fjq19SX3Kyw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In the presence of TGF-β, HATGF-βRII-Δcyt–transduced CTLs continue to proliferate and grow normally in long-term cultures.
HATGF-βRII-Δcyt–transduced, nontransduced, and eGFP-transduced CTLs were harvested 2 weeks after transduction. From each group, 1 × 106 cells were stimulated weekly with LCLs and fed twice weekly with IL-2 ± 5 ng/mL TGF-β. The figures show results from one representative experiment. (A) This panel represents CTL cell numbers (× 106) recorded from weekly cell counts in the 3 CTL groups grown without TGF-β (■) and with TGF-β (▴). (B) This panel shows 3[H] thymidine uptake in nontransduced CTLs (black) and HATGF-βRII-Δcyt–transduced CTLs (gray) before addition of TGF-β then on days 4 and 12.
