![Fig. 1. Transduced CTLs surface express HA tag of mutant TGF-βRII receptor. / Cells of an EBV-specific CTL line 14 days after retroviral transduction with SFG:eGFP were stained with either PE-labeled anti-IgG1 (A) or anti-CD3 antibodies (B). CTLs transduced with SFG:HATGF-βRII-Δcyt (truncated TGF-βRII containing HA tag) gene were stained with FITC-labeled donkey antirabbit antibody alone (isotype control) (C). Nontransduced (D) or HATGF-βRII-Δcyt–transduced CTLs (E,F) were then stained with anti-HA monoclonal antibody followed by incubation with FITC-labeled donkey antirabbit antibody and PE-labeled CD8 or CD4 antibody. Six weeks after sorting CTLs for the HA tag, HA expression was measured on CD8+ cells (G [isotype control] and H). Surface immunofluorescence was analyzed by flow cytometry.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3179/6/h80922475001.jpeg?Expires=1724246760&Signature=QeEByMlwHgpsP2OW8Em-DboOXcNRkO0s-UtW6IHz1FahosgD7D3hrheDYh7Cc3I12HufL1jDvK4bbXEy7ima0KYaPqGJFKK8pW9QbO2ozRsw4KXDaKyDVh9wMeYeFINVQ~bHLIbL-G5Au5K4P9Jd9ZxDyqQ0MM7~SGbWoih8NLYJPiWvXrv46kViNG6yiSnousi067jcHjBFn8voJXFH3~U-VLSCd2akIJZP~~CkPU0LBFRWPyyJrjkbXBQ8d2lqBPPwQ6nr972qQzaAvi6F6NUaMsEGBXkqSEMbLcakddnuHpbJP3U09qUKLY6lpPSPqew5UaZu35rr5Qmj8pkamA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transduced CTLs surface express HA tag of mutant TGF-βRII receptor.
Cells of an EBV-specific CTL line 14 days after retroviral transduction with SFG:eGFP were stained with either PE-labeled anti-IgG1 (A) or anti-CD3 antibodies (B). CTLs transduced with SFG:HATGF-βRII-Δcyt (truncated TGF-βRII containing HA tag) gene were stained with FITC-labeled donkey antirabbit antibody alone (isotype control) (C). Nontransduced (D) or HATGF-βRII-Δcyt–transduced CTLs (E,F) were then stained with anti-HA monoclonal antibody followed by incubation with FITC-labeled donkey antirabbit antibody and PE-labeled CD8 or CD4 antibody. Six weeks after sorting CTLs for the HA tag, HA expression was measured on CD8+ cells (G [isotype control] and H). Surface immunofluorescence was analyzed by flow cytometry.
