Fig. 2.
Fig. 2. CD28-independent expression of CTLA4 and absence of B7 receptors other than CD28 and CTLA4 in permeabilized P1CTL T cells. / (A) CD28-independent expression of intracellular CTLA4 in activated P1CTL. (B) CD28-independent expression of intracellular CTLA4 in tumor-infiltrating P1CTL. The genotype of CD28 locus (+/−or −/−) and the antibodies (CTLA4 or ctrl for isotype control) used for panels A and B are shown in the legends in panel B. (C, D) Blocking of B7- immunoglobulin binding to activated T cells by anti-CD28 or anti-CTLA4. Activated CD28+/− P1CTL were incubated first with either anti-CD28 or anti-CTLA4 mAb ascites. They were then incubated with biotinylated B7 immunoglobulin or a murine IgG2a mAb isotype control (ctrl). The amount of biotinylated B7 immunoglobulin or ctrl was determined by PE-conjugated streptavidin. Data in panels A, C, and D were repeated at least 5 times, and those in panel B were repeated twice.

CD28-independent expression of CTLA4 and absence of B7 receptors other than CD28 and CTLA4 in permeabilized P1CTL T cells.

(A) CD28-independent expression of intracellular CTLA4 in activated P1CTL. (B) CD28-independent expression of intracellular CTLA4 in tumor-infiltrating P1CTL. The genotype of CD28 locus (+/−or −/−) and the antibodies (CTLA4 or ctrl for isotype control) used for panels A and B are shown in the legends in panel B. (C, D) Blocking of B7- immunoglobulin binding to activated T cells by anti-CD28 or anti-CTLA4. Activated CD28+/− P1CTL were incubated first with either anti-CD28 or anti-CTLA4 mAb ascites. They were then incubated with biotinylated B7 immunoglobulin or a murine IgG2a mAb isotype control (ctrl). The amount of biotinylated B7 immunoglobulin or ctrl was determined by PE-conjugated streptavidin. Data in panels A, C, and D were repeated at least 5 times, and those in panel B were repeated twice.

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