Enhancement of megakaryocytic and erythrocytic differentiation in CD34⁺ cells by EphB4.

CD34⁺ cells were transduced with MSCV-GFP (left) or MSCV-EphB4 (right). GFP⁺ cells were then sorted and cultured in the presence of SCF, IL-3, and IL-6 for 7 days. Two-color FACS was performed for the expression of CD41-phycoerythrin and CD14-APC or the expression of glycophorin A-PF (gpA-PE) and CD33-APC. Data shown are from 1 representative experiment of 5 or 6 experiments with data summarized in the text.