Differential effects of PAF and PMA on tyrosine phosphorylation of p100-PPA and MAPK in CHO-αIIbβ3-PAFR cells.

Cells were incubated with either 100 nM PAF for 2 minutes or 100 nM PMA for 30 minutes. Then 1 mg/mL Fg was added and cells were plated onto BSA-precoated wells. After 15 minutes, cells were lysed and proteins (50 μg) were resolved by SDS-PAGE, transferred to PVDF membranes, and probed with antiphosphotyrosine mAb PY99, as described in “Materials and methods.”