Flow cytometric assessment of the PAF-induced aggregation of CHO-αIIbβ3-PAFR cells.

Either CHO-αIIbβ3-PAFR (A) or CHO-αIIbβ3 cells (B) (2.5 × 10⁶/mL) were incubated for 15 minutes with soluble Fg (0.5 mg/mL) in the presence or absence of 100 nM PAF and then fixed with 0.25% paraformaldehyde. Forward (FS) and side (SS) light scatter data of each sample were analyzed by flow cytometry as described in “Materials and methods.”