Fig. 4.
Fig. 4. Megakaryocytic progenitor assay. / Bone marrow cells (1 × 105) were cultured with MegaCult-C media in the presence of 10 ng/mL IL-3, 20 ng/mL IL-6, and 50 ng/mL TPO according to the instruction manual. After 7-day culture, the colonies formed were stained with AChE. Results represent the mean (±SD) number of CFU-Meg colonies per femur in 2 independent experiments.

Megakaryocytic progenitor assay.

Bone marrow cells (1 × 105) were cultured with MegaCult-C media in the presence of 10 ng/mL IL-3, 20 ng/mL IL-6, and 50 ng/mL TPO according to the instruction manual. After 7-day culture, the colonies formed were stained with AChE. Results represent the mean (±SD) number of CFU-Meg colonies per femur in 2 independent experiments.

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