Time course study of the effect of TGF-β₁and SDF-1 on the cell cycle progression and frequency of apoptotic cells in cultured CD34⁺ CB cells.

Primary cultures of lin⁻ CB cells were set up in SFM containing 5 GFs and then, after 4 days, CD34⁺ cells were isolated and transferred to secondary cultures containing SF and TPO (both at 50 ng/mL) either without (control) or with TGF-β₁ (5 ng/mL), or SDF-1 (100 ng/mL), or both. Values shown are the mean ± SEM of measurements made after PI staining of cells from 3 independent experiments. (A) Proportion of CD34⁺ cells in G₀/G₁. (B) Proportion of apoptotic cells.