HLA-DQ5 HY CTL recognized DBY amino acid sequence PHIENFSDIDMGE.

The DBY peptides indicated were added to irradiated HLA-DQ5⁺ female PBMCs. After incubation of 24 hours at 37°C, HLA-DQ5 HY CTLs were added; 16 hours later, supernatant was harvested and IFN-γ content was measured. Two peptides containing the common amino acids PHIENFSDIDMGE were recognized by the HLA-DQ5 HY CTL.