Fig. 2.
![Fig. 2. IL-7 treatment induces proliferation of CD45RA+CD45RO− naive cells without CD45RO up-regulation. / (A) CD45RA+CD45RO− T cells were isolated and left untreated or treated with IL-7 (1000 U/mL), IL-2 (100 U/mL), or PHA (4 μg/mL). PHA cultures were also treated with IL-2 (20 U/mL). The cells were analyzed by flow cytometry at day 0, 2, 4, and 6 for CD45RA+CD45RO− expression. Values shown represent at least 6 donors. (B) CD45RA+CD45RO− T cells were isolated and left untreated or were treated with IL-7 (1000 U/mL) or PHA (4 μg/mL). On day 6, the cells were pulsed with 1 μCi3[H]thymidine and cultured for an additional 6 hours prior to analysis by means of a scintillation counter. Values are the median of 5 different donors run in triplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3310/6/h80922495002.jpeg?Expires=1724231303&Signature=gbFYKJp02YRTFoHowtQNWhU0EW0CRpVfIgLHR3RRNhjLGBzfY~gyEqlPMIIPHuCcQ0KCrO59YRicKJ19-FPMNl~nScnNFywW1b8XLVWZGN-HZXn9ScsRlEm2bAkjYnM35udrg3MVGR2N55nIaHsDQYc4FkoIS45F9Q2D7V4sFJwIXbESwSUAMeJdeELtInFryEuOlcmiKvTyu3DqqFimHcMKPB--7taDacFCkhQipRHotXhTo8ER7iEWbPptSOC8-pUP23mvrR4WhZkCMh5oy8-XrAW3XQ5tuzxbgsjAe3UXHKdySWNbDJUDN7OWvrHi5rzgKaZDSWwBR9d2jXehcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-7 treatment induces proliferation of CD45RA+CD45RO− naive cells without CD45RO up-regulation.
(A) CD45RA+CD45RO− T cells were isolated and left untreated or treated with IL-7 (1000 U/mL), IL-2 (100 U/mL), or PHA (4 μg/mL). PHA cultures were also treated with IL-2 (20 U/mL). The cells were analyzed by flow cytometry at day 0, 2, 4, and 6 for CD45RA+CD45RO− expression. Values shown represent at least 6 donors. (B) CD45RA+CD45RO− T cells were isolated and left untreated or were treated with IL-7 (1000 U/mL) or PHA (4 μg/mL). On day 6, the cells were pulsed with 1 μCi3[H]thymidine and cultured for an additional 6 hours prior to analysis by means of a scintillation counter. Values are the median of 5 different donors run in triplicate.
