Increase in cytogenetic aberrations in Y⁺ primary transplanted G3 mTerc^−/− BM cells.

Spectral karyotyping was performed on metaphases of transplanted wildtype and G3 mTerc^−/− B6 BM male cells after 5 months of transplantation into female myeloablated hosts. (A) Image of a typical mTerc^−/− metaphase after SKY analysis showing a robertsonian translocation between chromosomes 5 and 6 (classified colors). (B) Corresponding reverse 4′6-diamidino-2-phenylindole·2HCl (DAPI) image of the same metaphase. (C) Complete karyotype of the same metaphase showing the chromosomes by spectral, DAPI, and classified images, respectively. (D) Examples of the clonal t(5;6) translocation in G3 mTerc^−/− B6 BM cells.