Reduced ability of sDCs to stimulate T-cell proliferation when cultured with anti–IFN-α/β.

The sDCs (96% CD11c⁺) isolated from B6 mice were cultured for 18 hours in the presence (circles, dotted lines) or absence (squares, solid lines) of anti–IFN-α/β immunoglobulin, washed, irradiated, and used as APCs for TCR transgenic T cells. (A) Different numbers of sDCs and 10⁵ CD8⁺ responder T cells purified from 2C TCR transgenic mice were added to wells with (open symbols) or without (solid symbols) antigenic peptide (Ser-Ile-Tyr-Arg-Tyr-Tyr-Gly-Leu at 0.05 nM). (B) Different numbers of sDCs and 10⁵ CD4⁺ responder T cells purified from DO11.10 TCR transgenic mice were added to wells with (open symbols) or without (solid symbols) antigenic peptide (OVA aa 323 through 336 at 144 nM). (C) Five thousand sDCs were added to wells with 10⁵ CD8⁺responder T cells purified from 2C TCR transgenic mice and differing amounts of specific peptide. (D) Five thousand sDCs were added to wells with 10⁵ CD4⁺ responder T cells purified from DO11.10 TCR transgenic mice and differing amounts of specific peptide. Each of the panels shown here is representative of results obtained in at least 3 independent experiments.