Histology of systemic organs from BM-transplanted GS mice.

Organs from PPCA^−/−mice transplanted with total −/−BM transduced with the MSCV-PPCA (BMT-PPCA) retrovirus were isolated at different time points after treatment. Hematoxylin and eosin-stained tissue sections of the liver (LIV), kidney (KID), and spleen (SP) from a BMT-PPCA–treatedPPCA^−/−mouse killed at 9 months after treatment, and from age-matched wild-type andPPCA^−/−mice revealed the complete restoration of normal tissue morphology with BM expressing PPCA, compared to the extensive vacuolation present in thePPCA^−/−control mouse. Size bar corresponds to 30 μm.