PLZF inhibits GATA-2–dependent reporter activity in transient transfection assays.

293T cells were transfected with a luciferase reporter plasmid containing 2 copies of double GATA sites in the mouse CD34 promoter (0.5 μg), together with expression plasmids for GATA-2 (GATA-2/pMT2, 200 ng; solid bars) and the indicated amounts of PLZF/pSG5 plasmids. Luciferase activities were standardized against renilla luciferase activity from a cotransfected control reporter (pRL-CMV–renilla luciferase) and were expressed as fold increases of the activity of reporter alone.