American Society of Hematology

Fig. 6.

$Fig. 6. The coiled-coil domain of Stat5 induces homodimerization of Stat5-RARα fusion proteins and binding to SMRT. / (A) Radiolabeled Gal4-Stat5-RARα proteins and SMRT were precipitated with GST (−) or GST–Stat5(1-331) (B) fusion proteins and the bound radiolabeled fraction was compared to 10% input (I). Binding of Stat5(1-636)–RARα and Stat5(1-333)–RARα, but not RARα, to GST–Stat5(1-331) is seen, indicating that the coiled-coil domain mediates homodimerization. Furthermore, stable interaction of radiolabeled SMRT and GST–Stat5(1-331) can also be observed.$

The coiled-coil domain of Stat5 induces homodimerization of Stat5-RARα fusion proteins and binding to SMRT.

(A) Radiolabeled Gal4-Stat5-RARα proteins and SMRT were precipitated with GST (−) or GST–Stat5(1-331) (B) fusion proteins and the bound radiolabeled fraction was compared to 10% input (I). Binding of Stat5(1-636)–RARα and Stat5(1-333)–RARα, but not RARα, to GST–Stat5(1-331) is seen, indicating that the coiled-coil domain mediates homodimerization. Furthermore, stable interaction of radiolabeled SMRT and GST–Stat5(1-331) can also be observed.

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