Fig. 3.
Stat5-RARα fusion proteins enhance transcriptional repression.
293T cells were transfected in triplicate with 0.75 μg of the indicated pCMV-Gal4 fusion plasmids, 1.5 μg of 2 times UAS-TK-luciferase plasmid, and 1 μg of a promoterless renilla luciferase plasmid by calcium phosphate coprecipitation. Cells were stimulated with ATRA (10−7 M and 10−6 M) and repression or induction is given relative to the luciferase activity obtained by the DNA-binding domain of Gal4 alone. Transcriptional repression is given as the mean and SD of 5 experiments.
