Fig. 2.
Stat5-RARα fusion proteins containing the coiled-coil domain block hematopoietic differentiation but respond to TSA treatment.
U937 (A), HL60R (B), and HL60 cells (C) were transduced with retroviral vectors coexpressing the indicated Stat5-RARα fusion proteins and EGFP. In U937 cells, coexpression of EGFP and CD14 was detected by FACS 48 hours after induction with vitamin D3 (10−6 M) and TGF-β (5 nM). HL60R and HL60 cells were differentiated with ATRA (10−6 M) for 5 days before measuring coexpression of EGFP and CD11b. (*P < .008, **P < .03). (D) HL60R cells transduced with the indicated vectors were treated with ATRA (10−6 M) for 5 days with or without TSA (120 nM) and then assayed for coexpression of EGFP and CD11b.
