Titration of TH9402 concentration for specific depletion of host-reactive T cells.

Spleen cells from C57BL/6 mice were first primed with BALB/c spleen cells in a 5-day mixed lymphocyte culture. The primed cells were then treated with photodynamic cell purging process using different concentrations of TH9402. After treatment, the cells were cultured with irradiated BALB/c or BCL1 stimulator. After 2 days, cytotoxicity against corresponding targets was tested in a standard 4-hour chromium release assay. Cytotoxicity against irrelevant targets was tested to ensure the specificity of the killing. The values represent mean plus SD of triplicates. This is a representative sample of 3 different experiments.