Cells expressing wild-type PAI-2 have reduced expression of CD-14.

The level of expression of the monocytic marker CD14 in THP-1 cells transfected with pCI-neo (░) or in cells expressing wild-type (■) or mutant PAI-2 (▧) was assessed by flow cytometry using an anti-CD14 antibody. Levels of CD14 expression in nontreated cells expressing wild-type PAI-2 were 50% lower than in control cells (pCI-neo–transfected) or in cells expressing mutant PAI-2. PMA treatment of cells resulted in an increase in CD14 expression in all cell groups; however, CD14 levels remained lower in cells expressing wild-type PAI-2 compared with control and mutant PAI-2–expressing cells.