American Society of Hematology

Fig. 7.

$Fig. 7. Endogenous u-PA activity is inhibited in THP-1 cells expressing wild-type PAI-2. / (A) Zymographic analyses of u-PA activity in cytoplasmic extracts prepared from nontransfected THP-1 cells (lane 2) or 2 representative clones of THP-1 cells transfected with pCI-neo (THP-1/neo; lanes 3 and 4), THP-1 clones (a and c) expressing wild-type pCI–PAI-2 (THP-1/PAI-2wt; lanes 5 and 6), and THP-1 clones (f and h) expressing mutant PAI-2 (THP-1/-PAI-2Ala380; lanes 7 and 8). Recombinant u-PA was used as a positive control (lane 1). (B) Subcellular localization of the u-PA activity in the various THP-1 cell lines. The u-PA activity was localized to the particulate fraction (lanes 5, 6, 8) but was not detected in the particulate fraction of cells transfected with wild-type PAI-2 (THP-1/PAI-2wt; lane 7). No u-PA activity was detected in either the cytosolic (lanes 1-4) or nuclear fraction (lanes 9-12) of any THP-1 cell line.$

Endogenous u-PA activity is inhibited in THP-1 cells expressing wild-type PAI-2.

(A) Zymographic analyses of u-PA activity in cytoplasmic extracts prepared from nontransfected THP-1 cells (lane 2) or 2 representative clones of THP-1 cells transfected with pCI-neo (THP-1/neo; lanes 3 and 4), THP-1 clones (a and c) expressing wild-type pCI–PAI-2 (THP-1/PAI-2wt; lanes 5 and 6), and THP-1 clones (f and h) expressing mutant PAI-2 (THP-1/-PAI-2_Ala380; lanes 7 and 8). Recombinant u-PA was used as a positive control (lane 1). (B) Subcellular localization of the u-PA activity in the various THP-1 cell lines. The u-PA activity was localized to the particulate fraction (lanes 5, 6, 8) but was not detected in the particulate fraction of cells transfected with wild-type PAI-2 (THP-1/PAI-2wt; lane 7). No u-PA activity was detected in either the cytosolic (lanes 1-4) or nuclear fraction (lanes 9-12) of any THP-1 cell line.

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