Analysis of bone marrow B-cell lineage development.

Flow cytometric analysis in Eμ-A1 transgenic mice (shaded bars) and wild-type littermates (open bars) using the convention devised by Hardy et al.29 Flow cytometry data were gated as described “Materials and methods.” Data are presented as the mean (± SD) percentage of the B220⁺ lymphocyte population for 4 mice of each genotype. Statistically significant differences (P < .05) were found in fraction B (subset of pro–B cells) and fraction D (pre–B cells), signified by asterisks.