Effect of LY294002 on TPA- and IL-4–induced survival on B-CLL lymphocytes.

B-CLL lymphocytes from 3 representative patients were preincubated in the presence (▪) or absence (■) of 20 μM LY294002 for 1 hour before the addition of drugs. After 48 hours of cell culture, cell viability was analyzed by flow cytometry. Results are represented as the mean ± SD from one experiment performed in duplicate.