Rapid termination of CSF-1R signaling in ICSBP^−/− BMMs.

BMMs were deprived of CSF-1 for 16 hours following stimulation with 200 ng/mL CSF-1 at 37°C for the indicated time points. Total extracts were subjected to SDS-PAGE and western blot analyses with an antiphospho-Erk-1/2 antibody (upper panel). To confirm equal protein loading the membrane was stripped and blotted with an anti–Erk-1/2 antibody (lower panel).