Fig. 8.
BH3 peptide–induced cell death and intracellular calcium in erythrocytes.
Induction of cell death by the BH3 peptide increases erythrocyte intracellular calcium. (A) Intracellular calcium levels as determined by the Fluo-3 AM probe. Erythrocytes cultured in the presence and absence of plasma were loaded with the calcium probe Fluo-3 AM and analyzed by flow cytometric analysis immediately following initial cell culture and at 4 and 6 days respectively. The thin line represents erythrocyte autofluorescence. Fluo-3 fluorescence is represented in bold. (B) Intracellular calcium levels in the presence of Ant-BH3 and Ant-BH3-A78. Fluo-3 fluorescence is represented over time prior to and following addition of the peptides.
