Bak-BH3 peptides and erythrocyte cell death.
Bak-BH3 peptides induce erythrocyte cell death. (A) First, 5 × 106 erythrocytes were cultured in SFM and treated with 50 μM Ant, Ant-BH3, or Ant-BH3-A78 or left untreated. Cell number was determined at 0, 24, and 48 hours for triplicate cultures and is represented as mean cell number ± SD. (B) Following 24 hours of treatment, samples were taken from panel A cultures for flow cytometric analysis of annexin-V FITC binding (bold). The thin line again represents erythrocyte autofluorescence in the absence of annexin-V FITC. (C) First, 5 × 106erythrocytes were cultured in SFM in the presence and absence of 10% PPP. Cells were then treated with 50 μM Ant or Ant-BH3. Cell number was determined at 0 and 24 hours, again for triplicate cultures. Results are presented as mean cell number ± SD and represent the 24-hour time point.
