Fig. 3.
Bcl-XL expression and isolation of contaminating reticulocytes.
(A) Erythrocytes were labeled with the RNA probe thiazole orange and analyzed by flow cytometry to identify the RNA-containing subset of cells prior to its depletion from the erythrocyte sample by cell sorting. The top panel represents unlabeled cells; thiazole orange–labeled cells are represented in the bottom panel. The erythrocytes selected for retention for further analysis are gated and represent 96.04% of the total cell population. (B) Western blot analysis of the expression of Bcl-XL in an erythrocyte sample before and after depletion of thiazole orange–staining cells.
