Cytokine expression by PD-F4 cells.

PD-F4 cells coexpressed cytokines. Expression of IFN-γ, IL-2, and IL-4 was examined by flow cytometric analysis in PD-F4, which was stimulated with autologous BLCLs (panels A-C), no stimulators (panels G-I), or allogeneic BLCLs (panels J-L) for 5 hours in the presence of BFA. A gate was placed on CD4⁺ events. Panels D-F are for PD-F4 stimulated as for panels A-C, but stained with respective control isotype antibodies. A representative of 5 independent experiments is shown.