Reconstitution and hematopoietic differentiation capacity of Pax5^−/− pre-B I cells upon serial in vivo transplantation and in vitro expansion.

Flow cytometric analysis of bone marrow (panel A) and thymus (panel B) 3 weeks after reconstitution with a primary GFP⁺Pax5^−/− pre-B I cell clone. Flow cytometric analysis of bone marrow (panel C) and thymus (panel D) 3 weeks after transplantation of a quarternary GFP⁺Pax5^−/− pre-B I cell clone. Flow cytometric analysis of reconstitution of the γδ TCR-expressing thymocyte, the NK T cell, NK cell, and CD8⁻ and CD8⁺ DC compartments in the thymus (panel E) and of the myeloid compartment (gated on GFP-expressing cells) in the spleen of a fifth serial GFP⁺Pax5^−/− clone-transplant recipient that lacks αβ TCR-expressing thymocytes (panel F).