DNA array hybridization determination of chemokine mRNA expression levels in rhesus macaque spleen tissues during SIV infection.
Total RNAs were purified from snap-frozen spleen samples obtained at necropsy and pooled according to disease state. Complementary DNA labeled with 33P–deoxycytidine triphosphate and reverse transcribed from each pool was hybridized to the human cytokine arrays, washed, and exposed to a high-resolution phosphorimaging screen. (A) The indicated values for each chemokine mRNA represent the mean signal intensity per pixel for the duplicate spots, after subtraction of local background and normalization against the mean signal intensity per pixel for 9 housekeeping genes (B). The uninfected pool included macaques M5600 and M6600; the acute-infection pool included M5499, M5699, M0999, M5899, and M5999; and the AIDS pool included M1799 and M5199. The new nomenclature for chemokines3 is shown here as the second part of each name.
