CD83⁺ IDC in lymph nodes are increased during acute SIV infection but decreased during AIDS.

(A-B) Lymph node cell suspensions were labeled and gated for expression of CD83 and lack of CD20 to identify IDCs. (A) Proportion of CD83⁺ IDCs in unseparated lymph nodes from normal, healthy monkeys (N, n = 5), and monkeys with acute SIV infection (A, n = 7), or AIDS (n = 5). IDCs are increased in acute SIV infection (P < .05) and decreased during AIDS (P = .05) as compared to normal, healthy animals. Each circle represents a different animal, and horizontal bars represent the mean. (B) Representative dot plots of lymph node cells from healthy monkeys and monkeys with acute SIV infection or AIDS; 400 000 events are shown to highlight IDCs (R1). (C-K) CD83 expression in lymph nodes was analyzed using in situ hybridization for CD83 RNA (C-E) and immunohistochemistry for CD83 protein (F-K) in healthy monkeys (C,F,I), and monkeys with acute SIV infection (D,G,J), or AIDS (E,H,K). Infrequent CD83⁺ IDCs in monkeys with AIDS are highlighted by arrows. F indicates follicle. Ab staining was detected using streptavidin-peroxidase with diaminobenzidine substrate. Tissues were counterstained with eosin (C-E) or hematoxylin (F-K). Original magnification × 100 (C-E), × 200 (F-H), × 600 (I-K).