CD83 expression identifies activated IDCs in lymph nodes of healthy monkeys.

Lymph node single-cell suspensions were stained with mAbs to CD20 and CD83 and a panel of cell surface markers. (A) Dot plot of CD83 versus CD20. IDCs (R1) express high levels of CD83 and lack expression of CD20. (B) Phenotype of CD83⁺ IDCs gated on R1. Fluorescence due to isotype control mAb was limited to the first decade. Data are representative of 12 experiments.