Fig. 4.
Interactions of STAT5b-RARα, PML-RARα, and PLZF-RARα and wild-type RARα/RXRα with the CoR SMRT and CoA TRAM-1.
(A) The in vitro translated proteins indicated on the left were incubated with or without SMRT or TRAM-1 as indicated and the radiolabeled RARE, DR5G. The location of the complex containing the chimeric or wild-type receptor plus SMRT/TRAM-1 is indicated by the solid triangle; the location of the chimeric or wild-type receptor alone is indicated by the open triangle. (B) SDS-PAGE and autoradiography were performed on in vitro translated and35S-methionine–labeled PLZF, RARα, or STAT5b alone or following incubation with SMRT and immunoprecipitation with SMRT antibody as indicated.
