Detection of bcr/abl protein expression and tyrosine phosphorylation in tissues from diseased and healthy mice.

(A) Cells (10⁶) from each indicated mouse tissue were lysed in a final volume of 100 μL and proteins were resolved with 6% SDS-PAGE. An α-Abl Western blot was performed with mAb 24-11 to detect the expressed bcr/abl proteins. (B) Similarly prepared protein lysates were subjected to 9% SDS-PAGE and immunoblotted using the antiphosphotyrosine mAb 4G10. A longer exposure is presented at the bottom to show proteins with weak signals. The bcr/abl proteins are indicated by ■ at the right of each band. The molecular weight standards are shown in kilodaltons on the left. Note that lane 7 (1-63 bcr/abl no. 12 spleen) and lane 11 (Δ[1-63] bcr/abl no. 1 thymus) are derived from “healthy” mice. The other tissues had tumor involvement.