Development of immature and mature lymphoid-derived DCs (DC2) after intrathymic injection of CD34⁺ cells in RAG2^−/− γc^−/−mice with a human thymus transplant.

Immunohistochemistry and confocal microscopy were performed 6 weeks after intrathymic injection of CFSE-labeled sorted CD34⁺CD38⁻ fetal liver cells using CD123-PE and unconjugated CD11c mAbs. CFSE-labeled cells show green dots. CD123-PE–positive cells are red (visualized by rabbit anti-PE and Cy3-conjugated goat antirabbit), and CD11c⁺ cells visualized by goat antimouse biotin and streptavidin-Cy5 are blue. CFSE-labeled cells that express CD123 alone (green dotted red cells), CD11c alone (green dotted blue cells), and CD123 and CD11c (green dotted purple cells) are visible. Isotype controls were negative. Original magnification × 400.