Development of immature and mature DCs after intrathymic injection of CD34⁺ cells in mice.

Immunohistochemistry and confocal microscopy were performed 6 weeks after intrathymic injection of CFSE-labeled sorted CD34⁺CD38⁻ fetal liver cells (A) and 4 weeks after the injection of MACS-purified CD34⁺ fetal liver cells (B) using CD123-PE and unconjugated CD83 mAbs. CFSE-labeled cells show green dots. CD123-PE–positive cells are red (visualized by rabbit anti-PE and Cy3-conjugated goat antirabbit) and CD83⁺ cells, visualized by goat antimouse biotin, and streptavidin-Cy5 cells are blue. CFSE-labeled cells that express CD123 alone (green dotted red cells), CD83 alone (green dotted blue cells), and CD123 and CD83 (green dotted purple cells) are visible. Isotype controls were negative. Original magnification × 800 (A) and × 400 (B).