Surface expression of CD16 is required for CD38-mediated increases in protein tyrosine phosphorylation.

YT CD16⁺ and YT mock cell lines were resuspended in serum-free RPMI-1640 medium and incubated with the appropriate mAb. Cells were then lysed, subjected to SDS-PAGE (10% gel under reducing conditions), transferred to PVDF membranes, and immunoblotted with RC20-HRP, an antiphosphotyrosine mAb. The same number of cell equivalents was loaded in each well. Molecular mass markers are indicated in kilodaltons. Data are from 3 independent experiments.