Matrigel analysis of PCLP-1⁺CD45⁻ cells incubated with or without M-CSF.

After coculture of PCLP-1⁺CD45⁻ cells with or without M-CSF in the presence of bFGF and OSM, adherent cells were replated onto Matrigel, and the photograph was taken after 12 hours of incubation. Arrowheads indicate the sprouting of cell cytoplasm. Note that cellular networks are more frequently found in the culture of PCLP-1⁺CD45⁻ cells that were incubated with OP9 stromal cells in the presence of M-CSF.