Effect of soluble Flt-1 on hematopoiesis in AGM culture.

On the second day of culture, sFlt-1 virus was inoculated. On the next day, medium was changed to normal growth medium. Nonadherent cells were harvested after 10 days of incubation and were plated onto methylcellulose culture in the presence of IL-3, IL-6, and EPO. Note that sFlt-1 significantly increased the number of GEMM. Error bar shows standard deviation. Double asterisk indicates P < .01 (Student t test). This experiment was repeated 2 times, and generally the same results were obtained. Virus vectors used are Mock, pMIG vector, sFlt-1, pMIG-sFlt-1.