HbF and HbA in bone marrow erythropoiesis.

HbA and HbF (left panel) and mRNA (right panel) content during erythropoiesis in vivo. Bone marrow mononuclear cells from 3 healthy adults were labeled and sorted in the independent experiments as shown on Figure 5. CD71⁺GPA⁻ cells versus CD71⁺GPA⁺ were compared with respect to HbF and HbA protein content (A) (HPLC measurements), and γ-globin mRNA and β-globin mRNA levels (B) (quantitative PCR). The mean values, SD bars, and paired t test values are shown.