HbA and HbF and mRNA content during the course of erythropoiesis in vitro.

(A-C) Hemoglobin protein levels were quantitated by HPLC. (A) Mean cellular content of HbF. (B) Mean cellular content of HbA. (C) HbF/HbA ratios. (D-F) Globin transcript levels (molecules/1 ng total RNA) were quantitated by real-time PCR. (D) Mean content of γ-globin mRNA transcript. (E) Mean content of β-globin mRNA transcripts. (F) Mean mRNA ratios of γ/β globin. The initial levels above background for hemoglobin (panels A and B) and globin mRNA (panels D and E) are shown with arrows. The asterisks and P values are noted on the days when increases above the initial hemoglobin and globin mRNA values (shown with arrows) became statistically significant.