Fig. 3.
Pulse-chase experiments of Cys38Arg and WT factor XI.
Transfected cells were radiolabeled by 35S-methionine and then chased by cold methionine for 1 to 20 hours. Cell lysates were precipitated by monoclonal antibodies against factor XI, electrophoresed on gradient 4% to 12% SDS-PAGE gels under reducing conditions, and exposed to autoradiography. Note that Cys38Arg was produced similarly to WT and did not undergo accelerated degradation.
