Immunohistochemical analysis of pS473-AKT in MM patients.
(A-C) Bone marrow biopsies obtained from MM patients demonstrate immunopositivity for pSer473-AKT in plasma cells. Biopsies were fixed in 10% zinc formalin for 2 hours, subsequently decalcified in Decalcifier II (Surgipath Medical Industries, Richmond, IL) for 2 hours and processed by a standard automated processor. Four μM sections mounted on glass slides underwent microwave antigen retrieval process in citrate buffer, subsequently incubated with anti–pSer473-AKT antibody at 1/100 dilution overnight at 4°C similar to described previously.9 Slides were then stained with a streptavidin/peroxidase detection system.9 Note that panels A and C illustrate prominent nuclear and nucleolar staining in the neoplastic plasma cells. Magnification × 500.
