Fig. 10.
In vivo priming of male antigen (H-Y) cytotoxicity.
Female mice were primed intravenously with 1.5 × 105male DCs from each subset and 20 days later splenocytes were restimulated in vitro. After 5 days the indirect measure of DNA fragmentation was used to assess CTL-mediated apoptosis in male B6 Con A blast targets using the JAM assay.33 Immunizing populations were as follows: open circles, CD4−/CD8− DCs; closed circles, CD4+ DC; squares, CD8α+ DC; and crosses, HBSS-injected controls. Similar levels of lysis of female targets required 10- to 100-fold higher doses of CTLs induced by each subset (not shown). Error bars show means of triplicates ± SD.
