Stimulation of peptide-specific T cells by DC subsets.

Equal numbers of FACS-sorted DCs from each of the subsets were aliquoted and pulsed with different concentrations of NP68 peptide. DCs were then used as stimulators for F5 T-cell (5 × 10⁴/well) proliferation at 4 × 10⁴(A) or 4 × 10³ (B) DCs/well. Error bars show means of triplicates ± SD. The indicated DC numbers represent only a guide because they are the input cell number, rather than those DCs present following DC loss by adherence to the tube and loss following the washing steps. Due to the low DC numbers present for each peptide concentration, recounting was not feasible.