Plaque-reduction neutralization assay showing the effect of Gal(+)-virus incubation with anti-Galα1-3Gal–containing human serum.
Gal(+) and Gal(−) virus supernatants, diluted to 103PFU/mL, were incubated with a human O-type reference serum, lacking measles-specific Ab and previously diluted 1:5 in PBS. Similarly, diluted Gal(+) and Gal(−) virus supernatants were incubated with the same serum, previously either decomplemented by heat-inactivation, or incubated with Galα1-3Galβ1-4GlcNAc or A trisaccharides. Subsequently, virus/serum mixtures were analyzed for viral titers by a plaque-reduction neutralization assay on Vero cells. Results (mean SEM) in pentuplicates are shown as the mean percentages as compared to the untreated (PBS) Gal(+) and Gal(−) virus titers (100%). ND denotes experimental conditions involving H virus that were not determined.
