Fig. 4.
![Fig. 4. Corroboration of differential mRNA expression by quantitative real-time RT-PCR. / Real-time RT-PCR curves for 4 different genes (leukocyte proteinase 3, ubiquitin protein-ligase [UBCH10], DAPK1, and caspase 4) with the respective GAPDH controls are displayed. A representative BM-CD34+ sample is shown on the left, and a representative PB-CD34+ sample is shown on the right side. Analysis of each sample was performed in duplicate. The differences of the CPs (ΔCP) of target and GAPDH are indicated. The smaller the ΔCP, the higher the relative abundance of the target's mRNA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/6/10.1182_blood.v99.6.2037/6/h80622274004.jpeg?Expires=1724276444&Signature=sIxojxIUFDwQFB~SamzfJD5EfMS7JNHNahFGyBt1ibDYniDLDxhO7B5nchu7Ag1Ge2BItBDu-xDAnkDtIWrTQgcYnKzd6G9fedyKXnNy~QYQxnGCetsAoYOlqrBuEtRlKFts8q5P57sFpvSt2Ag0BYJ~YGhit-BoS9xsTUoiZ8fOu7J4fUAdyy68AKfdq-MCvComEFUCyJoTfJGxfgzzOYa7TKLv79pYrXS8IiueGZ4sWYCdle7ZxfiTpQUIJDA0TD6lT2MbD5SoQlbKF3jhpx3YAMyBEJWQOhP46FYlLDhlbSLhbBoMEzu17--~fjBp3Yi5jEmaG5IepGvtewza4Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Corroboration of differential mRNA expression by quantitative real-time RT-PCR.
Real-time RT-PCR curves for 4 different genes (leukocyte proteinase 3, ubiquitin protein-ligase [UBCH10], DAPK1, and caspase 4) with the respective GAPDH controls are displayed. A representative BM-CD34+ sample is shown on the left, and a representative PB-CD34+ sample is shown on the right side. Analysis of each sample was performed in duplicate. The differences of the CPs (ΔCP) of target and GAPDH are indicated. The smaller the ΔCP, the higher the relative abundance of the target's mRNA.
