Fig. 5.
IL-12–producing capacity of DC populations from mice treated with cytokines.
Sorted subpopulations of splenic DCs (0.5 × 106/mL) from cytokine-treated mice were stimulated in culture with CpG-ph (0.5 μM), IL-4 (100 U/mL), IFN-γ, (20 ng/mL), and GM-CSF (200 U/mL). The FL data are from treatment with muFL, but treatment with huFL yielded similar results. Supernatants were harvested after 16 to 20 hours and then analyzed by ELISA for production of IL-12 p40 and p70. Data are from 1 experiment; error bars represent the range of results with duplicate samples. Similar results were obtained in more than 5 experiments using DCs from mice treated with ProGP-1 and FL and in 3 experiments using DCs from mice treated with G-CSF and pGM-CSF.
